Batman/Bruce Wayne ( Kevin Conroy): Wealthy industrialist whose parents were killed when he was a young boy. His guilt and desire for vengence has brought about Batman, an alterego who pursues justice through means outside the system.
Alfred Pennyworth (Efrem Zimbalist Jr): Bruce Wayne's butler, surrogate father and accomplice in his pursuits as Batman.
Dick Grayson/Robin (Loren Lester): Orphaned when he was a young boy when his parents were killed in a circus accident. Bruce Wayne took him in, having faced similar circumstances when he was a boy. He eventually allows Dick to join him in Batman's pursuits of justice.
Commissioner James Gordon (Bob Hastings): He's long been a source of information for Bruce Wayne and Batman. Bruce Wanye and his daughter, Barbara, have long been close friends.
Dr. Leslie Thompkins (Diana Muldaur): A confidant of Bruce Wayne's. She is like a surrogate mother in whom he confides some of his most personal internal conflicts.
Det. Harvey Bullock (Robert Costanzo): A hard-nosed street cop who sees Batman as nothing more than a vigilante who is as bad as the criminals that he pursues.
Barbara Gordon/Batgirl (Melissa Gilbert): Daughter of Commissioner James Gordon and a close friend of Bruce Wayne.
The Joker (Mark Hamill): Batman's archenemy. He is brilliant, but not completely sane.
Harley Quinn (Arlene Sorkin): The Joker's girlfriend. She gleefully assists him in his crazy schemes.